October 3, 2022
@
12:00 PM
–
1:00 PM
Super-resolution and hyperspectral imaging with spatio-temporally modulated illumination light
Randy A. Bartels, PhD
Professor of Electrical and Computer Engineering and Biomedical Engineering
Colorado State University
co-sponsored by the Morgridge Institute for Research and the UW SPIE/Optica Chapter
Coherent nonlinear microscopic imaging is a powerful method for studying specimens without the introduction of external labels. Nonlinear optical imaging is particularly useful in complex environments that suffer from significant optical scattering and absorption where conventional camera-based methods fail. Optical imaging with single pixel detection enables imaging detailed structures in specimens by scanning a point focus of light in three dimensions. Simple point scanning provides limited information relative to the bandwidth of a single pixel detector. I will discuss recent advances in multiplexed imaging where dynamically structured illumination light is able to extract significantly more spectroscopic and spatial information from a specimen. Application of dynamic light structuring to super resolution microscopy, hyperspectral imaging, and Raman microscopy will be discussed.
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