October 2, 2023
@
12:00 PM
–
1:00 PM
Applications of fluorescence lifetime measurements in flow cytometry
Jessica P. Houston, PhD
Professor, Chemical & Materials Engineering Department
New Mexico State University
Co-sponsored by Morgridge Institute for Research and the UW SPIE/Optica Chapter
New Location : Engineering Hall 1227
Abstract:
Methods for high throughput single-cell analyses have become quite complex over the last decade with emerging technologies that advance the speed of imaging and sorting as well as enhance the number of parameters that can be measured from a single cell. Many instruments, cytometers, or similar devices provide essential features about cells because optical measurements provide not only spatial but also temporal information about the intracellular environment. Time-resolved flow cytometry (TRFC) is one form of cytometry that captures temporal information about fluorescent molecules inside the cell. Such information does not rely on brightness and often correlates to signaling events, molecular movement, and dynamics of molecular interaction. Various TRFC technologies will be presented as well as applications that focus on metabolic mapping of tamoxifen resistant breast cancer cells using autofluorescence. Focus will also be placed on a chip-based cytometer that utilizes acoustic focusing for more accurate fluorescence lifetime measurements. The long-term impact of this work is to develop new tools that provide more quantitative fluorescence information at the throughput of a flow cytometer.
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